You are here: ERA-PG / File repository / Call 2006 / / Multistress
Title Multiple Stress Responses and Adaptations
   
Acronym MultiStress
   
Duration 1 July 2007 - 1 July 2010
   
Project leader John Mundy, University of Copenhagen, Denmark
   

Project partners

 Søren Brunak, Danish Technical University, Denmark
Tapio Palva, University of Helsinki, Finland
Paolo Costantino, Universita Roma La Scapienza, Italy
Ida Ruberti, Università di Roma, Italy
Dick Vreugdenhil, Wageningen University, The Netherlands
Alte M. Bones, Norwegian Universtiy of Science and Technology, Norway
  
Funding Danish Agency for Science, Technology and Innovation (DASTI), Denmark 
   The Academy of Finland (AKA), Finland
   Ministry of Education, University and Research (MIUR), Italy
  The Research Council of Norway (RCN), Norway
   Netherlands Genomics Initiative / Netherlands Organisation for Scientific Research (NGI/NWO), The Netherlands
   
Total granted budget  € 1,788,397
   
Abstract  

The general objectives are to analyze responses and adaptations of plants to multiple stresses and to identify the level and functions of stress regulatory networks and crosstalk. Both biotic (pathogens & insects) and abiotic (unfavourable environmental conditions) stresses will be taken into account. Genomic approaches in the model Arabidopsis will be used to provide knowledge and tools for breeding crops with enhanced tolerance to stress conditions in the field.
 
Output from the project (publications, patents, data resources, etcetera)

Progress
In addition to the publications in 2008-09 below, the partners are collaborating on a transcriptomic analysis of 10 ecotypes and 15 mutants or transgenic over-expressor lines exposed to 4 stress combinations. A portion of a replicate plant set prior to stress treatments is shown below. Given replicates and controls, 1650 mRNA samples were initially isolated. Given the available funds, an optimized experimental design includes 600 samples currently being analyzed with Agilent array technology.

Publications (related to MultiStress since 2008)
-Autophagic components contribute to hypersensitive cell death in Arabidopsis. Hofius D, Schultz-Larsen T, Joensen J, Tsitsigiannis DI, Petersen NH, Mattsson O, Jørgensen LB, Jones JD, Mundy J, Petersen M. Cell. 2009 May 15;137(4):773-83.

-Arabidopsis MAP kinase 4 regulates gene expression through transcription factor release in the nucleus. Qiu JL, Fiil BK, Petersen K, Nielsen HB, Botanga CJ, Thorgrimsen S, Palma K, Suarez-Rodriguez MC, Sandbech-Clausen S, Lichota J, Brodersen P, Grasser KD, Mattsson O, Glazebrook J, Mundy J, Petersen M. EMBO J. 2008 Aug 20;27(16):2214-21.

-Arabidopsis mitogen-activated protein kinase kinases MKK1 and MKK2 have overlapping functions in defense signaling mediated by MEKK1, MPK4, and MKS1. Qiu JL, Zhou L, Yun BW, Nielsen HB, Fiil BK, Petersen K, Mackinlay J, Loake GJ, Mundy J, Morris PC. Plant Physiol. 2008 Sep;148(1):212-22.

-A genetic framework for the control of cell division and differentiation in the root meristem. -Dello Ioio R, Nakamura K, Moubayidin L, Perilli S, Taniguchi M, Morita MT, Aoyama T, Costantino P, Sabatini S. Science. 2008 Nov 28;322(5906):1380-4.

-Auxin regulates Arabidopsis anther dehiscence, pollen maturation, and filament elongation. Cecchetti V, Altamura MM, Falasca G, Costantino P, Cardarelli M. Plant Cell. 2008 Jul;20(7):1760-74.

-Modulation of intracellular proline levels affects flowering time and inflorescence architecture in Arabidopsis. Mattioli R, Marchese D, D'Angeli S, Altamura MM, Costantino P, Trovato M. Plant Mol Biol. 2008 Feb;66(3):277-88.

-The Arabidopsis homeodomain-leucine zipper II gene family: diversity and redundancy. Ciarbelli AR, Ciolfi A, Salvucci S, Ruzza V, Possenti M, Carabelli M, Fruscalzo A, Sessa G, Morelli G, Ruberti I. Plant Mol Biol. 2008 Nov;68(4-5):465-78.

-Embedding mRNA stability in correlation analysis of time-series gene expression data. Farina L, De Santis A, Salvucci S, Morelli G, Ruberti I. PLoS Comput Biol. 2008 Aug 1;4(8):e1000141.

-Contribution of omega-3 fatty acid desaturase and 3-ketoacyl-ACP synthase II (KASII) genes in the modulation of glycerolipid fatty acid composition during cold acclimation in birch leaves. Martz F, Kiviniemi S, Palva TE, Sutinen ML. J Exp Bot. 2006;57(4):897-909.

-Involvement of CBF transcription factors in winter hardiness in birch. Welling A, Palva ET. Plant Physiol. 2008 Jul;147(3):1199-211.

-System-wide molecular evidence for phenotypic buffering in Arabidopsis. Fu J, Keurentjes JJ, Bouwmeester H, America T, Verstappen FW, Ward JL, Beale MH, de Vos RC, Dijkstra M, Scheltema RA, Johannes F, Koornneef M, Vreugdenhil D, Breitling R, Jansen RC. Nat Genet. 2009 Feb;41(2):166-7.

-Integrative analyses of genetic variation in enzyme activities of primary carbohydrate metabolism reveal distinct modes of regulation in Arabidopsis thaliana. Keurentjes JJ, Sulpice R, Gibon Y, Steinhauser MC, Fu J, Koornneef M, Stitt M, Vreugdenhil D. Genome Biol. 2008;9(8):R129.

- Quantitative genetics in the age of omics. Keurentjes JJ, Koornneef M, Vreugdenhil D. Curr Opin Plant Biol. 2008 Apr;11(2):123-8.

-The FRO2 ferric reductase is required for glycine betaine's effect on chilling tolerance in Arabidopsis roots. Einset J, Winge P, Bones AM, Connolly EL. Physiol Plant. 2008 Oct;134(2):334-41.

-Towards global understanding of plant defence against aphids--timing and dynamics of early Arabidopsis defence responses to cabbage aphid (Brevicoryne brassicae) attack. Kusnierczyk A, Winge P, Jørstad TS, Troczynska J, Rossiter JT, Bones AM. Plant Cell Environ. 2008 Aug;31(8):1097-115.

-A mixture model approach to sample size estimation in two-sample comparative microarray experiments.
Jørstad TS, Midelfart H, Bones AM. BMC Bioinformatics. 2008 Feb 25;9:117.