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Title Genomic research-assisted breeding for sustainable production of quality grapes and wine
   
Acronym GRASP GRAPE WINE
   
Duration 1 July 2007 - 1 July 2010
   
Project leader Eva Zyprian, Julius Kühn Institute (JKI), Germany
   

Other project participants

Serge Delrot, CNRS, Bordeaux, France
Nathalie Chiosne, INRA, Evry, France
Didier Merdinoglu, UMR 1131 INRA-University of Strasbourg, Colmar France
Charles Romieu, UMR 1083 INRA-ENSAM-University of Montpellier, France
 Nicolas Bernard*, SFERIS - SIANEO [Company], Villeneuve-les-Maguelone, France
Joachim Kopka, Max Planck Insitute for Molecular Plant Physiology, Golm, Germany
Stella Grando*, IASMA, Trento, Italy
Rob Verpoorte*, Leiden University, The Netherlands
Manuel Pedro Salema-Fevereiro, ITQB/Universidade Nova de Lisboa, Portugal
Maria Solame Soares Pais, ICAT, Portugal
Sara Amâncio, Institutio Superior de Agronomia, Portugal
Hans-Jörg Böhm*, PLANSEL Lda (JBP) [Company], Portugal
Jose Miguel Martinez- Zapater, CNB - CSIC, Madrid, Spain
Juan Carreño Espin, IMIDA, La Alberca, Spain
Fausto Carillo, I.T.U.M., Blanca [Company], Spain
  
Funding National Institute for Agricultural Research (INRA), France 
   Research Centre Juelich – Project Management Juelich (FZJ-PTO) on behalf of the Federal Ministry of Education and Research (BMBF), Germany
   Foundation for Science and Technology (FCT), Portugal
  Ministry for Education and Science MICINN), Spain  
   
  *participant with own funding
   
Total granted budget  € 1,546,496
   
Abstract  

Grapes are currently grown on 3.6 Million ha in Europe (EU 25) and amount to a total yearly production of app.185 Million hl wine production (2004) in the European Union (OIV 2004). Europe is also an important producer of table grapes with Italy, Spain and Greece producing close to 2 Million tons of the total 12 Million tons world production in 2004 (USDA 2004). The concerns of consumers for improved food quality produced with environmentally safe and sustainable agriculture demands the development of cultivars of table- and wine grapes improved in natural pathogen resistance and fruit quality to reduce the extensive use of fungicides and ensure high-quality production. Demand of viticulturists for quality varieties adapted to changing environmental conditions must be anticipated in the context of global climatic changes.
Breeding of grapevines is a long-lasting task and urgently requests novel tools to achieve these aims efficiently. The fundamental goal of this proposal is to identify the gene sequences and mutations responsible for phenotypic variation of resistance and berry quality traits in grapevine through the combination of genomic, functional genomic, metabolomic and quantitative genetical methods. It will link the Europan research activities in this area and construct a common platform for compilation and exploitation of Vitis phenotypic, metabolomic, transcriptomic and genetic/genomic data.
 
Progress

Figure 1 Infecting healthy grapevine plants with Erysiphe necator (powdery mildew) by gentle touching with highly infected leaves from a susceptible cultivar for analysis of differential gene expression of candidate genes in resistant and susceptible grapes (Zyprian, Siebeldingen)
Figure 2 A visiting scientist from ICAT (Portugal) performing infections with Plasmopara viticola (downy mildew) on susceptible and resistant grapevine plants by spraying a solution of fungal spores to the lower leaf surface at Geilweilerhof in Germany (Zyprian, Siebeldingen)		  

Figure 3 Studying the fleshless flb mutant to understand fruit development for the best adaptation to environmental conditions (Romieu, Montpellier)
Figure 4 Studying gene expression along berry development to understand quality (Martinez-Zapater, Spain) 		  
 
Project website

http://urgi.versailles.inra.fr/projects/GRASP/
 
Output from the project (publications, patents, data resources, etcetera)

Presentations

Zyprian, E.,' GRASP Grapevine Genome research assisted breeding for sustainable Production of quality grapes and wine.' ERA-NET PG Grant holders meeting, 3-6 October 2007, Tenerife, Spain

Zyprian, E., Rex, M., Töpfer, R., 'GRASP Grapevine Genome research assisted breeding for Sustainable Production of quality grapes and wine', ERA-NET PG GABI cooperative project, 2008, GABI Statusseminar Potsdam, Germany

Zyprian, E., 'GRASP GRAPE WINE Genomic-assisted breeding for sustainable production of quality grapes and wine', ERA-NET PG GABI cooperative project, GABI Status Seminar, Potsdam, Germany, March 2009

Ali, K., Maltese,^ F., Choi, Y. H., Zyprian, E., Rex, M., Verpoorte, R., 'Metabolic Characterization of Different Grapevine (/Vitis
vinifera/) Cultivars using ^1 H NMR and Multivariate Data Analyses', Young Scientists Meeting, Phytochemical Society of Europe, Murcia, Spain, May 2009

Fortes, A. M., Silva, M. S., Ali, K., Maltese, F., Choi, Y. H., Sousa, L., Martínez-Zapater, J. M., Verpoorte, R., Pais, M. S., 'Transcriptional and metabolic profiling during ripening of Trincadeira grapes', COST meeting (INPAS), Tallinn, Estonia, May 2009